Developments

The laboratory “Molecular Biology”

Research fellows of the laboratory have developed a unique test system for routine DNA diagnosis of hereditary forms of deafness in Yakutia.

The invention in the field of medical genetics can be used in otorhinolaryngology for non-syndromic hereditary deafness diagnosis (NHD). The method of molecular genetic diagnostics of NHD detects 17 disease-caused mutations in the GJB2 and GJB6 genes by the polymerase chain reaction (PCR)-amplification of the corresponding parts of genes. It is carried out in 8 reaction mixtures using specific primer pairs. The amplicons are analyzed either without being preliminary broken up with endonucleases (for determining mutations c.312del14, c.333-334delAA, GJB6-D13S1830, GJB6-D13S1854), or after the hydrolysis of specific restriction endonucleases (for determining mutations IVS1 +1 G> A, c.35delG, c. 71G> A, c.79G> A, c.167delT, c.235delC, c.224G> A, c.299-300delAT, c.360delGAG, c.341A> G, c.269T> C, c.101T> C, c.109G> A). It provides an accurate and objective diagnosis of hereditary autosomal recessive deafness.

Patent № 2448163 “A method of detection 17 mutations in the GJB2 and GJB6 genes in non-syndromic hereditary deafness”. 

Authors: N.A. Barashkov, L.U. Jemileva, O.L. Posukh, S.A. Fedorova, E.K. Khusnutdinova E.

The laboratory “Mechanochemical Biotechnology”

According to the proposed method, natural antioxidant, namely dihydroquercetin, is added to the juice. The antioxidant is added in the ratio of 15-19 mg per l liter of liquid product. This method allows to increase the shelf life of freshly squeezed, concentrated and reconstituted juice as well as nectars and juice drinks, because Dihydroquercetin prevents the reactions of free radical and peroxide oxidation, fermentation and mold growth.

The main reason for juice souring is free radical and peroxide oxidation caused by active forms of oxygen released during the activity of microorganisms. It results in high concentrations of organic acids and acidification of liquid food. Therefore, to increase the period of storage the most active natural antioxidant, Dihydroquercetin, should be used.

Patent № 2436419 “A method of increasing the shelf life of juice”.

Authors: V.V. Anshakova, A.A. Shein, E.S. Khlebny, M.M. Shashurin, M.A. Zhukov, B.M. Kershengolts

The invention is for the food industry. The three-phase bioproduct NANOYAGEL -M in the form of nanostructured powder is added to juice, milk and other food. This powder contains supramolecular complexes of biologically active substances and is obtained fr om raw lichen throuhg mechanochemical technology. It is added in the amount of 50-100 mg / l of liquid product. It increases the shelf life of freshly squeezed, concentrated and reconstituted juice as well as nectars, juice drinks, milk and milk products. The fact is that biologically active substances contained in the bioproduct NANOYAGEL-M have antibacterial and antifungal effect. On the other hand, they prevent from free radical and peroxide oxidation.

Patent № 2437582 “A method of increasing the shelf life of juice, milk and other liquid food with the help of mechanochemical bioproduct NANOYAGEL-M”

Authors: V.V. Anshakova, B.M. Kershengolts, M.A. Zhukov

The invention is related with biotechnology (biopharmacy), namely, obtaining a sorption material from raw plant material for medical purposes (therapeutic and prophylactic).

One of the main areas of biotechnology involves the development of sorption materials and their further application for enterosorption in medicine and the medical industry. In comparison with hemosorption the method of enterosorption has a number of advantages. Firstly, there is no need for surgical intervention and consequently, there are no complications typical for hemosorption; secondly, there is no direct contact with body fluids (blood or lymph); and lastly, sorption therapy can be widely used in a patient’s treatment both in the field and home conditions.

Patent № 2437582 “A method of producing sorption material from lichen thalli”

Authors: B.M. Kershengolts, A.S. Sharina, E.V. Karataeva, V.V. Anshakova

The invention is related with biotechnology (biopharmacy), namely, the production of a biological agent with actoprotective effect. It increases physical endurance and reduces the period of adaptation (both to physical activity and changing environmental conditions) due to the rapid elimination of lactate, without increasing body weight. This biologically active supplement can be used to increase physical activity, endurance and recovery of sportsmen as well as to improve health of people leading an active lifestyle.

Today sport is characterized by an extremely high level of physical and mental stress on the body. Modern athletes are adapted to intense training and competitive pressures by applying a number of educational, psychological and biomedical techniques. Currently, the level of training results in further stress growth on the body. Therefore, the main purposes are to enhance the physical performance of athletes, prevent fatigue and speed recovery. It is especially important for high-class athletes since physical and mental stress that accompanies their preparation and participation in competitions often leads to functional disruptions of the organism. In this regard, a fundamental task is the optimization of the training process by introducing new highly efficient natural products which can provide the body's adaptation to constantly increasing stresses and ensure better results.

Patent № 2477143 “A Herbal Biologically Active Actoprotective and Adaptogenic Supplement and the Method of Preparation” "

Authors: V.V. Anshakova, B.M. Kershengolts

The invention is related with the food industry, namely, the production of bread and bakery products from wheat flour and wheat and rye flour with natural food supplements. The mechanochemical food additive from lichen thalli “Yagel-T” (“reindeer moss – T”) enriches bakery products with essential micronutrients and nonhormonal physiologically active substances and promotes to their absorption. It also improves bread physical and chemical characteristics, preserves its freshness and extends the shelf life.

The production of bakery products with the addition of cereals, wheat bran, malt extract, grain mixes, cumin, flax, sunflower, coriander and sesame seeds, iodized salt, prunes, raisins, fructose, kelp powder  and all kinds of dressings has been organized. Moreover, dietary products recommended for people suffering from diabetes are also made, for example, the Karelian bread from a mixture of rye (10%) and second-class wheat (85%) flour with the addition of fermented rye malt, sugar, syrup, dried grapes, anise and coriander.

Patent № 2466542

Authors: B.M. Kershengolts, E.V. Karataeva, V.V. Anshakova

The invention is related with biotechnology (biopharmacy), namely, the production of biological products having bactericidal action for medical purposes. The antibiotic drug from the lichen thalli raw is produced without solvents in a single process step with the help of mechanochemical technologies. They allow to form β- oligosaccharides (an active filler) on account of cleavage of some β- glycosidic bonds in lichen β- polysaccharides, and a complex between an active filler and pharmakon. Thus it increases bioavailability of pharmakon and antibiotic activity of the drug 5-10 times. The proposed method and properties of the bio-complexes differ favourably from those of the analogues produced according to classical technology.

Lichens of the genus Cladonia and Cetraria are medicinal plants used in folk medicine to treat many diseases. In lichen thalli there are up to 80 % of carbohydrates, half of which is represented by β- gomopolisaccaride, liсhenin and isolichenin having immunomodulatory, anti-tumor  and hepatoprotective properties.

Patent № 2466542

Authors: B.M. Kershengolts, V.V. Anshakova

The laboratory “Polymer Nanocomposites Technologies”

The invention is related with polymeric materials, namely, the development of antifriction polymeric composites which can be used for bearings and other friction units operated at high loads and sliding speed.

There are composite materials on the base of polytetrafluoroethylene (PTFE) and different chemical inorganic fillers. They are used for in the production of sliding bearings, mechanical seals and other friction units. The materials are self-lubricating and antifrictional with low friction coefficient, but they cannot be applied under high loads and sliding speeds due to insufficient durability, which reduces their working life.

A high wear-resistant material is characterized by an increased friction coefficient which significantly lim its its application.

The invention can reduce the friction coefficient of the PTFE based composite material while maintaining excellent wear resistance.

The material made on a standard equipment allows to increase durability 1.50-1.75 times and reduce the friction coefficient 2-5 times, its prototype keeping the strength characteristics. Optimum filler content is 5-15 wt. %. A further increase in the filler content results in worse strength and tribological characteristics and a hihger friction coefficient.

Patent № 2454439

Authors: T.S. Struchkova, S.A. Sleptcova, A.A. Okhlopkova

The invention is related with polymer materials, namely, antifriction polymeric composite materials that can be used to make parts of friction units in machines and equipment: slide bearings, seals of rotary and reciprocating movement and others.

One of the promising methods of structural modification of polymers is the use of layered silicates as fillers. Silicates as effective modifiers of the polymer matrix can form continuous spatial structures in the system due to the structure of minerals. The modifying ability of layered silicates can be fully realized as a result of intercalation of polymer macromolecules in the interlayer space of the filler particles. Under the action of the polymer macromolecules the subsequent exfoliation (separation) of fillers into single layers, 1 nm thick, can form nanocomposites. They have the properties much better than those of the former polymer. The main objective of the development of the nanocomposite “polymer - layered silicate” is to provide compatibility and the intercalation process. The simplest and most effective way is mechanoactive treatment of layered silicates.

The aim is to improve wear resistance, reduce the friction coefficient while maintaining the thermo-mechanical properties of the PTFE-based composite material.

Patent № 2460742

Authors: T.S. Struchkova, A.A. Okhlopkova, S.A. Sleptcova, E.S. Afanasyeva